Part:BBa_K3912002
Template1
Template 1 and 2 makes a molecular switch together to reflect whether the tau protein exists in the reaction system. The reaction that takes place is Recombinase Polymerase Amplification(RPA), in which we use two primers, called Amplification F and Amplification R. Template 1 is the single-strand DNA that make a loop-stem structure with the Template 2 strand. It has the priming sequence and the loop. The loop contains Amp-F, the sequence of Amplification F (also the sequence of the probe), while the priming sequence is a over-hang strand at the 3’ end, acting as the primer at the start of the reaction. Other sequence of Template 1 pairs with Template 2. In the RPA reaction, when tau protein is absent, no reaction will start; but when tau is present, the protein will bind with the aptamer sequence on Template 2 and pull it away, turning the loop-stem structure into a linear structure, and the priming sequence will bind with Template 2, acting as the primer to make a double-strand DNA molecule with Template 2 strand as the template. And then the primer-binding sequence will exist on the prolonged Template 1 strand for Amplification R to make a prolonged Template 2 strand, with the binding sequence of Amplification F replicated from Amp-F on it, thus the complete double-strand DNA molecule is formed and the RPA reaction will continue.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 62
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